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FAIR-CT96-2069
The assessment of genetic purity in hybrid varieties of crops
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Contacts
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FAIR Area 4.1 - Reformed CAP
:
Plant Genetics
:
Vegetable Oil/Fat
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Proposal No: |
FAIR-CT96-2069 |
| Date Prepared: |
January 1998 |
| Source: |
Proposal Abstract |
Abstract: The objectives of the proposed project are:
- to develop where necessary, optimise and evaluate the use of DNA profiling
methods (microsatellites and AFLP) for plant variety identification and
registration and for hybrid purity assessment in a number of crops of interest
to European agriculture (maize, sunflower, and Brassica crops, primarily oilseed
rape and the vegetable white kale).
- to use maize as a model species for examining hybrids of various types and
for comparison with the recognised and widely used biochemical methods for
hybrid purity assessments.
- to use oilseed rape and kale as models for other Brassica crops where
hybrid varieties of different types are commonly produced (e.g. Brussels
sprouts, cabbage, cauliflower), to compare the results obtained with those from
existing biochemical /molecular methods and to examine the transportability of
techniques between species within the genus.
- to develop not only the necessary profiling technology, but also the
overall methodology required for the use of the techniques in the seed testing
and certification context and to devise an operational system. This would
involve consideration of both technical and statistical issues.
- to organise a series of 'ring tests' which would compare the data obtained
from the use of the optimised method(s) with a particular seed lot with those
obtained from existing biochemical analysis, where appropriate, and observed in
field plots of the same lot. Whilst this comparison would apply primarily to the
overall level of hybrid purity determined by the different approaches, it would
also include the examination of any 'off-types'. This would form part of the
assessment of an optimised operational system for seed certification.
- to develop databases of varietal DNA profiles and begin to examine the
potential applications of such techniques and databases to varietal
registration.
Hence overall the proposed research will promote collaborative research and
the development of harmonised and efficient systems for plant variety testing
throughout the EU, aiming to ensure the availability of high quality varieties
to the agricultural industry and consumers. It will provide tools for production
of higher quality raw materials which in turn will increase the efficiency of
the agri-industries within Europe. In addition, the DNA profiling systems can be
used readily to trace varieties (and the products derived from them) throughout
'total quality management' systems, from propagative material to the end
product, both within and between companies. This will also contribute to the
improvement of the quality of produce.
Contacts
Coordinator
EC Scientific Officer
