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[BioMatNet Database - FAIR Program] FAIR-CT96-1633
Genetic Engineering of Carotenoid Metabolism: a Novel Route to Vitamins, Colours and Aromas for the European Market
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To find similar Items, click on a keyword below:
Biological Conversion : Biotechnology : FAIR Area 1.2 - Green Chemicals and Polymers Chain : Fine Chemicals : Flavours/Fragrances : Pharmaceuticals/Cosmetics : Plant Genetics



Type of Project Shared Cost
Contract No FAIR-CT96-1633
Total Cost 1,833,969 ECU
EC Contribution 1,177,916 ECU
Start Date 01/12/96
Duration 36 Months

Genetic engineering of carotenoid metabolism: a novel route to vitamins, colours and aromas for the European market

Objectives

Technical Approach

Carotenoids are terpenoid pigments very widespread in nature. They are industrially used as highly safe colorants of food and cosmetic preparations. Beta-carotene is the dietary precursor of vitamin A. In developed countries we eat between 1 and 1,5 mg/day beta-carotene and in some third world countries this figure is below 0.5 mg/day. Carotenoid metabolites also play a role in aroma: the flavour of many plant products, such as tomato and saffron, is due to volatile derivatives of the xanthophyll oxidative metabolism. The market for purified carotenoids is growing rapidly and it already exceeds 100 million US $/yr. Over 90% of the market is covered by chemically synthesised carotenoids.

Expected Results

I. Direct Results

  • Cloning of genes encoding specialised steps in carotenoid metabolism, that would allow the engineering in crops or microorganisms of high value compounds (e.g. zeaxanthin, astaxanthin, crocetin, and picrocrocin).
  • Production, through genetic engineering, of high-added-value carotenoid metabolites in widely cultivated crops (tomato) and in filamentous fungi.
  • Improvement, through genetic engineering, of the beta-carotene (provitamin A) content of main crops (potato, rice).
  • Preliminary assessment of the nutritional and agronomic qualities of the engineered organisms.

II. Indirect Results

Data on the physiology of organisms with altered carotenoid content and on the bioavailability of engineered carotenoids.

Applications

Speciality chemicals, human nutrition, feed additives.

Results To Date

  • Genes encoding novel, specialised steps in carotenoid metabolism have been cloned
  • All gene constructs planned have been introduced in transgenic plants and fungi
  • Preliminary modification of the carotenoid metabolism has been obtained in two crops (rice and potato)
  • Data on the bioavailability of ß-carotene have been obtained


Genetic Engineering of carotenoid metabolism





Contacts

Coordinator

EC Scientific Officer

Participant

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Updated by CPL Press: 03/07/2007 - biomatnet@biomatnet.org

 


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