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FAIR-CT96-1625
Valorisation of rapeseed/sunflower lecithins, a by-product of seed oil, in cosmetics and fermentation industries: use of natural carriers of phospholipids overproduced by filamentous fungi growing on lecithin substrates
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Further Information
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Agricultural Residues
:
Biological Conversion
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Bulk Chemicals
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FAIR Area 1.2 - Green Chemicals and Polymers Chain
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Pharmaceuticals/Cosmetics
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Vegetable Oil/Fat
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Type of Project |
Shared Cost |
| Contract No |
FAIR3-CT96-1625 |
| Total Cost |
1,423,145 ECU |
| EC Contribution |
949,617 ECU |
| Start Date |
01/01/97 |
| Duration |
36 Months |
Valorisation of rapeseed/sunflower lecithins, a by-product of seed oil, in cosmetics and fermentation
industries: use of natural carriers of phospholipids overproduced by filamentous fungi growing on lecithin
substrates
Objectives
When lipid substrates are present in the growth medium of fungi, some proteins are overproduced
due to a stimulation of the secretory pathway. This is the case for lipid transfer proteins (LTP), natural
lipid carriers that are produced by Aspergillus strains growing on different lipid substrates.
Technical Approach
A goal of the proposed project is to study the capabilities of lecithins from European seed oil
(rapeseed and sunflower) to enhance protein secretion (LTP but also other enzymes) and biomass
production. In cosmetics, lecithins are used as liposomes and another goal of the project is to improve
liposome technology by using the LTP produced by fungi.
Expected Results
- improving use of European seed oil lecithins (rapeseed and sunflower) in fermentation and cosmetic
industries
- improving LTP and enzyme secretion in industrial fermentation
- development of a new market for lecithins in fermentation industries
- improving efficiency of liposome technology in cosmetic applications
- development of new cosmetic products combining liposomes and LTP
- improving scientific knowledge on the structure and function of lipid transfer proteins
Application
- Use of lipid substrates in fermentation industries.
- Use of LTP as carrier of hydrophobic/lipid molecules in cosmetic industries.
- Other sectors can use lecithins and LTP such as the food and pharmaceutical industries.
Results To Date
- Up-scaling (300 litre) of high density fermentation using lecithins as a carbon source for
Aspergillus oryzae
- Determination of an optimised fermentation protocol for the overproduction of lipid transfer proteins
at an industrial scale
- Obtention of rapeseed and sunflower lecithins from the corresponding crops and oils
- Rapeseed and sunflower lecithins are as efficient as soya lecithins as a carbon source for A.
oryzae and overproduction of lipid transfer proteins
- Determination of the best physicochemical conditions for the extraction of the 18 kDa lipid transfer
protein from A. oryzae at an industrial scale
- Lab-scale purification of the 18 kDa lipid transfer protein and obtention of specific antibodies
against this protein, as well as determination of a new procedure for industrial purification
- Isolation and characterisation of a cDNA coding for the 18 kDa lipid transfer protein
- Determination of the amino acid sequence of 18 kDa lipid transfer protein
- Determination of the localisation of the lipid transfer protein in fungal cell (Golgi and cell walls).
Valorisation of rapeseed/sunflower lecithins
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