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[BioMatNet Database - FP5 Quality of Life Programme] QLK3-1999-00004
Enhanced, intelligent processing of food and related wastes using thermo-philic populations (INTELLTHERMCONTROL)
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Biological Conversion : Bulk Chemicals : Quality of Life - 3. The Cell Factory



Contract No: QLK3-1999-00004
Project Type: RS (Research and Technological Development Project)
Start Date: 01-02-2000
Duration: 48 months
Total Cost:
EC Contribution: 1 414 358 EUR
Scientific Officer:

Abstract

This project is aimed at improving the viability and environmental sustainability of the European food industry, by using thermophilic microbial "cell factories" combined with knowledge-based control to achieve safer, cleaner, more energy-efficient bioremediation of the vast volumes of wastes produced. We shall achieve this by: a) developing generic knowledge-based protocols for thermophilic process design and operation; b) combining these protocols with advanced microbiological and process analysis and control techniques to operate bio-reactors containing thermophilic bioprocesses for the pathogen-free treatment of food industry and related wastes, with maximal energy recovery. The outcome will be industrially tested Knowledge-Based Control System software.

Objectives

  • To develop a Knowledge-Based Control System containing generic knowledge-based protocols for the design and operation of robust and versatile processes to transform complex waste streams, using "cell factories" of thermophilic populations of microorganisms.
  • To utilise means of advanced microbiological and process analysis and control to assist this protocol development and its use in the operation and evaluation of thermophilic, mixed-population bioprocesses for the pathogen-free treatment of food industry and related wastes, with maximal energy recovery, in a variety of reactors.

Description of the work

  • A number of food and related waste-waters/streams of significance for European environmental sustainability will be selected as feed materials. Their main components will be analysed and their biodegradability characterised.
  • A mixed-species thermophilic microbial population will be supplied by one of the partners for use throughout the project. This will be characterised, in terms both of its microbial species and of the "key" substrates, intermediates, and products involved in the various stages of the treatment process for each selected feed material, and their fluxes, using carbon flux analysis, flow cytometry, and other advanced techniques, and the results incorporated into a knowledge base.
  • The thermophilic population will be used to convert the wastes in both stirred-tank reactors and a novel cocurrent gas-liquid reactor. The biodegradation performances of both types of reactor will be studied, to yield data for the knowledge base and subsequent process modelling, and the potential for "engineered" species balances assessed.
  • Predictive process models of various levels of complexity will be developed, assessed, incorporated into a "model base", and further used to develop knowledge-based control schemes and protocols to achieve robust, "optimal" populations and performance for the treatment of each waste.
  • The knowledge and model bases will be combined with the control strategies and protocols into a Knowledge-Based Control System for thermophilic biotreatment of waste-waters.

Deliverables

The outcome of the project will be a software-based system for improved control and operation of thermophilic processes for food industry bioremediation, incorporating knowledge of the behaviour of microbial populations, and tested on a range of industrial waste-waters of importance throughout Europe. The protocol of the system will be adaptable for use with other populations and processes, thus making it one of a new generation of "generic" advanced control and management systems.





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