QLK5-1999-01389 BIOTECH CONTROL: Better management of process water systems by biotechnological identification and treatment of detrimental micro-organisms and metabolites

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Summary Information



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Biotechnology : Crops for Paper/Pulp : Integrated Crop Protection & Biological Control : Quality of Life - 5.3.2 The Forestry-Wood Chain

Contract No:	QLK5-1999-01389
Source:	Progress Report - January 2002 - Abstract

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Progress Report - January 2002 - Abstract

Objectives

The main aim of this project is to minimise the loss of quality and productivity in paper mills that may occur at increased rates of paper recycling, while simultaneously minimising freshwater consumption. To achieve this the project aims to develop advanced biotechnical tools for slime control and to adapt other technologies to the specific needs of the paper industry. To achieve this microorganisms relevant to slime production will be investigated using a method that does not require cell cultivation, but depends on rRNA analysis. This includes the design and development of specific 16S-rRNA oligonucleotide probes for slime-relevant microorganisms enabling detection directly at their site of action. At the same time a QCM-D technique based on a unique sensor technology will be developed and perfected for use in monitoring biofilm formation. This combined system will be used to identify relevant microorganisms and pinpoint slime formation and hence enable targeted counter measures to be developed in order to eliminate critical organisms. In addition this project will screen new dispersants and test industrially available enzyme mixtures as a means of controlling unavoidable slime formation without using hazardous biocides. When applied together with the proposed advanced monitoring systems, this strategy will help minimise environmental and health risks.

Results and milestones

So far the work carried out has been performed according to the planned time schedule. The main objectives, in respect of the stated deliverables and milestones, during the first year have been achieved as follows:

• Screening of paper mill samples has been successfully performed using the FISH (fluorescent in situ hybridisation) technique and existing rRNA probes. In this context three bacterial groups were found to be dominant in various slime samples from two paper mills.
• First isolates from slime samples were phylogenetically analysed and the first gene probes for slime-relevant bacterial groups were constructed which allowed a more precise monitoring of the slime samples. As a result a 16 rDNA clone library of slime relevant bacteria has been initiated.
• Using a dedicated questionnaire and specific data sheets relevant information was obtained concerning various parameters in paper mills.
• An adjustable bioreactor has been developed that can be used for simulating industrial environments.
• A monitor, based on the QCM-D technique has been developed, as a means of studying initial biofilm formation.

Benefits and Beneficiaries

Paper production is one of the most water intensive industrial processes and its effluents have a significant effect on aquatic ecosystems. There is a growing realisation within the industry, that a reduction in freshwater consumption and increasing waste paper recycling rates do in fact contribute to better company results. In response to economic and environmental pressures, mill managers in many regions of Europe have achieved a significant reduction in effluent discharge. However, highly closed water loops, increased paper recycling rates and the resulting problems of increased slime formation have created a host of problems related to the effects on machine runnability, corrosion, hygiene and product quality. Against this background, this project has been elaborated with the aim of minimising losses in quality and productivity and minimising freshwater consumption, resulting in a step-wise improvement of the management of slime problems experienced in the industry.