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AGRE-0017
Improvement of In Vitro Plant Cultures at Laboratory and Industrial Scales: Control of Gaseous Environment by Using Selective Membranes as Lids of Culture Containers |
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Improvement of In Vitro Plant Cultures at Laboratory and Industrial Scales: Control of Gaseous Environment by Using Selective Membranes as Lids of Culture Containers1 Febuary 1991 - 31 January 1992 |
Many types of plants, including ornamentals, horticultural crops, vegetables and fruit bushes, can be produced using techniques of cell and tissue culture, generally referred to as 'in vitro plant micropropagation'. However, the procedures used are labour intensive and costly. By examining the effects of changes in gases found in the headspace of containers sealed with membranes of varying gas permeability, a new type of container, for which a patent application has been submitted, has been designed. Results obtained at laboratory and industrial scales, with different species investigated throughout the complete propagation process (from multiplication to acclimatisation stages), show that by using containers sealed with suitable membranes it is possible to improve the multiplication, survival, growth and quality of plants and as a result decrease the contribution of labour costs per plant.
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